Conversion of the Pathogenic Fungus Colletotrichum magna to a Nonpathogenic, Endophytic Mutualist by Gene Disruption

Hygromycin-resistant transformants of the cucurbit pathogen Colletotrichum magna (teleomorph: Glomerella magna) were generated by restriction enzyme-mediated integration (REMI) transformation.A rapid pathogenicity assay involving watermelon (Citrullus lanatus) seedlings was developed and 14,400 REMI transformants were screened and assessed for thei

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Detection of potential biodeterioration risks for tempera painting in 16th century exhibits from State Tretyakov Gallery.

In this study, we investigated biodeterioration of materials used in tempera painting by analyzing the structure of the microbiome in ancient tempera paintings exhibited in State Tretyakov Gallery, Moscow, Russia.Samples were obtained from 16th-century paintings, including a grand Russian Orthodox icon "The Church Militant" (all exhibits were witho

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